Oecd Guidelines for the Testing of Chemicals

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1. Several methods are available for determining n-octanol/water partition coefficients (Pow values). Pow values in the logarithmic range -2 to 4 (occasionally up to 5) can be experimentally determined by the shake-flask method set out in OECD Guideline 107. POW values in the range log POW between 0 and 6 can be estimated using high performance liquid chromatography as set out in OECD Guideline 117. Whilst both these methods can be applied to ionisable compounds buffered to a pH at which the compound is unionised, they are of limited applicability to multiprotic substances. The method set out in this guideline allows for the pH-metric determination of log Pow values of for ionisable and multiprotic compounds in the range -2 to 7. This guideline is based on a proposal submitted by the Department of the Environment, Transport and the Regions of the United Kingdom. The proposal was further developed also based on the basis of existing methods described in literature (1)(2)(3)(4)(5)(6).

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تاریخ انتشار 2000